Regulatory
PR2 (short

Part:BBa_K2404003:Design

Designed by: Ryan Coates   Group: iGEM17_Cardiff_Wales   (2017-09-22)


PR2 shortened - a salicylic acid-induced promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 238
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 514


Design Notes

- We isolated genomic DNA from wildtype Arabidopsis thaliana

- We designed primers to amplify the PR2 promotor sequence.
iGEM47 PR2pF tttCGTCTCtCTCAggagCAAGATCGTCAACTGTAACC
iGEM48 PR2pR tttCGTCTCtCTCGcattGTTATGTTTCACCTTAATCACC

- We gel extracted the DNA and digested with BsmBI to introduce the plasmid into pSB1C3.

- This plasmid corresponds to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard.


Source

This part comes from Arabidopsis thaliana and was isolated using specific primers.

References